LOCUS JX560328 3123 bp DNA circular SYN 02-OCT-2014 DEFINITION Cloning vector pSEVA231, complete sequence. ACCESSION JX560328 VERSION JX560328.1 GI:410073675 KEYWORDS . SOURCE Cloning vector pSEVA231 ORGANISM Cloning vector pSEVA231 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3123) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3123) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7902 /dnas_title="pSEVA231-MAGE" /organism="Cloning vector pSEVA231" /mol_type="other DNA" /db_xref="taxon:1246706" primer_bind complement(4900..4923) /note="F24" regulatory 4930..5032 /regulatory_class="terminator" /note="T0" gene 5160..5975 /dnas_title="neo" /gene="neo" CDS 5160..5975 /dnas_title="neo" /gene="neo" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AFV59774.1" /db_xref="GI:410073676" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_feature 6007..6252 /note="oriT" gene complement(6261..6923) /dnas_title="rep" /gene="rep" CDS complement(6261..6923) /dnas_title="rep" /gene="rep" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59775.1" /db_xref="GI:410073677" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" regulatory 7797..7901 /regulatory_class="terminator" /note="T1" CDS complement(901..2748) /note="mutL E32K" /translation="" RBS 2749..2765 /note="RBS" variation complement(2653..2655) /note="E32K mutation" gene complement(2769..3446) /codon_start=exo /note="Exo" /note="color: #a6acb3" /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG FVFGEQWR" gene complement(3446..4228) /codon_start=bet /note="Bet" /note="color: #a6acb3" /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA" gene complement(4237..4530) /codon_start=gam /note="open reading frame#1" /note="color: #a6acb3" /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF ESLCIDHLQRHGASKKSITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV" promoter 4600..4644 /note="pL promoter" primer_bind 4857..4879 /note="ARCK1" gene complement(9..719) /codon_start=cI repressor /note="open reading frame#1" /note="color: #a6acb3" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG QSGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY EYPVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE SCSVVGKVIASQWPEETFG" primer_bind 75..94 /note="ARCK2" misc_feature 790..871 /note="primer for cro-pBBR1" /note="color: #a6acb3; direction: RIGHT" misc_feature 831..852 /note="final red retrieve" /note="color: #a6acb3" rep_origin 853..872 /note="pBBRI ORIGIN" /note="color: #a6acb3" PCR_primer 2659..2682 /note="mutL31S" PCR_primer 2639..2658 /note="mutL32F" ORIGIN 1 ttaattaagc caaacgtctc ttcaggccac tgactagcga taactttccc cacaacggaa 61 caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa aaacacctga 121 ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct ggctatgcag 181 aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc gtcaggaaag 241 cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag ccagaatgca 301 gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt ggtaaaggtt 361 ctaagcttag gtgagaacat ccctgcctga acatgagaaa aaacagggta ctcatactca 421 cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc tctggcgatt 481 gaagggctaa attcttcaac gctaactttg agaatttttg taagcaatgc ggcgttataa 541 gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc catccccatc 601 ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc ataaattgct 661 ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt tgtgctcata 721 cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga ctattttacc 781 tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg agatgtgtat 841 aagagacagc tggcctgccc ctcccttttg gttgggatcc tctagagtcT CATCGCCATT 901 TCACTCATCT TTCAGGGCTT TTATCGCCGG ATGTAAATCA ACAGATTGTA ACAGACCACC 961 CGGCGGCGTT TTCACAAGTT GCGGACATAA CCGTTCCACG TCCGCCAGCA GGGTTATGGC 1021 CTGTGCCATT GACCACTGCG CATGTTCGCT CATCAGATTT CGTGCAATCC ACTGCGCAAT 1081 ATTGCCAGGT TCGAATACGG ACTGCTTCGC CAGGTAGCCT ATCAGTTCAG GAATCAAGAT 1141 TTGTAAATTT TGTTGGCGTA AGGGTAAAGG CACTGCCCTG ATGGTCACAT GCTGTGCATC 1201 TGACTGGAAA TCAATACCCA ATTCCGCCAG GGCAGACTGC GCTTTTTCTA ATGCCGATTT 1261 TTCTTCGGCA GAAACTTTTA GCCGCAACGG AATCAGCAGC GGCTGGGCGC AAACGGGCGC 1321 TTCACCCGGC GTCAATTGTG CCTGACGCAG CCAACGTTCT GCCACTGGCA AGGATAAAAG 1381 TGAAATGTTG CCGTCGCGCT CCAGCAACGC ACAGTCGGAA TGGACGATAG TCAGTACCCG 1441 ACCAAAACTC TGACTGTTCG CCGCAAGTGC AGGTTCCTGC GGTTCCGGCG CTTTTAATTT 1501 TTGCATCGGC GCGGGCGTTT GCAAAAGCTG GCGATACACT TCACCTTGCT GTTTCTGGTA 1561 GCCTGGCTGC GCATTCGGCC AGGGGGCAGC CGGACGACTG CCTGATGCTG GCGCAGGAGT 1621 GTAGCGCGGA GCTACCGGCT CACGAGCTGC CGGTTCTGCA AAGTGATTGC GCCCCGCCGC 1681 CACGCGGTTT TCCGGAATGG AACGCGGTGC AGGTTGGGGT TCATCGTCCA GCGGTAGCGG 1741 CGTTTCCAGT TGCTGTTGTA GCACGCTCAG CACGCCCTGA TAGATAAAAT CATGCACCAG 1801 ACGCGACTGA TGGAAACGCA CTTCGTGTTT GGCGGGGTGC ACGTTGACGT CCACCTGATG 1861 TGGGTCGATC TCCAGATACA ACACAAATGC CGGTTGCTGA TCGGCCCCCA GTTTGTCTTC 1921 GCAGGCCTGG CGGATCGCGT GATTGATCAG GCGATCGCGC ATCATGCGAC CGTTCACGTA 1981 GCAATACTGA ATTTCTGCCA GTGCGGGCGT GGTGTGATTT GGATCGGCCA CCCAGCCGCG 2041 TAGCGTGAGA TCGCCGTGTT GCCATTCAAT CGCCAGCGCT TGTTCAAGAA AAGCGGTGCC 2101 GCAAATCGCG CCTAAGCGCC GTTCTTTTTG CCCGCCTTCC GGCACTGCGC GGTACTGACG 2161 CACAATTTTA CCGTTATGCG ACAGGTTGAT CGTGACGTCG AAACGCGCCA GCGCAATGCG 2221 GCGGATGATC TCATCAATGT GGTTAAATTC GGTTTTCTCG GTGCGCAGGA ATTTGCGCCG 2281 CGCCGGGGTG TTGTAGAACA GATCCAGCAC CTCCAGCGTC GTCCCCACAG GATGCGCCGC 2341 CGGTTTTACC GTCACGTTCA TATCGCGCCC TTCGGCATAG GCCTGCCAGG CTTCCTGCTG 2401 TTCTGCGGTG CGTGAAGTGA GCGTCAGGCG GGAAACCGAA CTGATACTCG CCAGCGCCTC 2461 ACCGCGAAAG CCCAGGCTGA TAATGGCTTC GAGATCGTCC AGAGAGGCGA TTTTACTGGT 2521 GGCATGACGA GCCAGCGCCA GCGCCAGCTC ATCTTTTTTG ATACCGCAGC CGTTATCACG 2581 AATGCGGATA AGTTTCGCCC CACCGCGTTC GATATCAATA TCGATACGCG TCGCACCTGC 2641 ATCGAGGCTG TTTTtCACcA GTTCTTTGAC TACCGACGCA GGTCGCTCGA CCACCTCACC 2701 TGCGGCAATC TGGTTCGCCA GTTGTGGCGG TAAGACCTGA ATTGGCATTT TGTAATCCTC 2761 CTCTCtcatc gccattgctc cccaaataca aaaccaattt cagccagtgc ctcgtccatt 2821 ttttcgatga actccggcac gatctcgtca aaactcgcca tgtacttttc atcccgctca 2881 atcacgacat aatgcaggcc ttcacgcttc atacgcgggt catagttggc aaagtaccag 2941 gcattttttc gcgtcaccca catgctgtac tgcacctggg ccatgtaagc tgactttatg 3001 gcctcgaaac caccgagccg gaacttcatg aaatcccggg aggtaaacgg gcatttcagt 3061 tcaaggccgt tgccgtcact gcataaacca tcgggagagc aggcggtacg catactttcg 3121 tcgcgataga tgatcgggga ttcagtaaca ttcacgccgg aagtgaactc aaacagggtt 3181 ctggcgtcgt tctcgtactg ttttccccag gccagtgctt tagcgttaac ttccggagcc 3241 acaccggtgc aaacctcagc aagcagggtg tggaagtagg acattttcat gtcaggccac 3301 ttctttccgg agcggggttt tgctatcacg ttgtgaactt ctgaagcggt gatgacgccg 3361 agccgtaatt tgtgccacgc atcatccccc tgttcgacag ctctcacatc gatcccggta 3421 cgctgcagga taatgtccgg tgtcatgctg ccaccttctg ctctgcggct ttctgtttca 3481 ggaatccaag agcttttact gcttcggcct gtgtcagttc tgacgatgca cgaatgtcgc 3541 ggcgaaatat ctgggaacag agcggcaata agtcgtcatc ccatgtttta tccagggcga 3601 tcagcagagt gttaatctcc tgcatggttt catcgttaac cggagtgatg tcgcgttccg 3661 gctgacgttc tgcagtgtat gcagtatttt cgacaatgcg ctcggcttca tccttgtcat 3721 agataccagc aaatccgaag gccagacggg cacactgaat catggcttta tgacgtaaca 3781 tccgtttggg atgcgactgc cacggccccg tgatttctct gccttcgcga gttttgaatg 3841 gttcgcggcg gcattcatcc atccattcgg taacgcagat cggatgatta cggtccttgc 3901 ggtaaatccg gcatgtacag gattcattgt cctgctcaaa gtccatgcca tcaaactgct 3961 ggttttcatt gatgatgcgg gaccagccat caacgcccac caccggaacg atgccattct 4021 gcttatcagg aaaggcgtaa atttctttcg tccacggatt aaggccgtac tggttggcaa 4081 cgatcagtaa tgcgatgaac tgcgcatcgc tggcatcacc tttaaatgcc gtctggcgaa 4141 gagtggtgat cagttcctgt gggtcgacag aatccatgcc gacacgttca gccagcttcc 4201 cagccagcgt tgcgagtgca gtactcattc gttttatacc tctgaatcaa tatcaacctg 4261 gtggtgagca atggtttcaa ccatgtaccg gatgtgttct gccatgcgct cctgaaactc 4321 aacatcgtca tcaaacgcac gggtaatgga ttttttgctg gccccgtggc gttgcaaatg 4381 atcgatgcat agcgattcaa acaggtgctg gggcaggcct ttttccatgt cgtctgccag 4441 ttctgcctct ttctcttcac gggcgagctg ctggtagtga cgcgcccagc tctgagcctc 4501 aagacgatcc tgaatgtaat aagcgttcat ggctgaactc ctgaaatagc tgtgaaaata 4561 tcgcccgcga aatgccgggc tgattagtaa tccggaatcg cacttacggc caatgcttcg 4621 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat 4681 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc agtatcaccg 4741 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgta 4801 tgttttttat atgaatttat tttttgcagg ggggcattgt ttggtaggtg agagatctga 4861 attgctatgt ttagtgagtg catgcaagct tgcggccgcg tcgtgactgg gaaaaccctg 4921 gcgactagtc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat 4981 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc 5041 aataattacg atttaaattt gtgtctcaaa atctctgatg ttacattgca caagataaaa 5101 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 5161 tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg 5221 cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt 5281 atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg 5341 tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc 5401 tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga 5461 ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg 5521 tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt 5581 ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg 5641 tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag 5701 aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc 5761 tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg 5821 gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc 5881 cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac 5941 tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg cggtccgcgc 6001 gttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata 6061 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg 6121 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg 6181 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg 6241 aggctggccg taggccggcc ctaccggcgc ggcagcgtta cccgtgtcgg cggctccaac 6301 ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc 6361 cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg 6421 ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc cggatacagg 6481 gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca 6541 accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg gccccacgcc 6601 acgcggtcat tgaccacgta ggccgacacg gtgccggggc cgttgagctt cacgacggag 6661 atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa agaacgtccg 6721 atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc catctgcgcc 6781 acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc ggcccacgcc 6841 tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc ttgaatgccg 6901 atttctctgg actgcgtggc catgcttatc tccatgcggt aggggtgccg cacggttgcg 6961 gcaccatgcg caatcagctg caacttttcg gcagcgcgac aacaattatg cgttgcgtaa 7021 aagtggcagt caattacaga ttttctttaa cctacgcaat gagctattgc ggggggtgcc 7081 gcaatgagct gttgcgtacc cccctttttt aagttgttga tttttaagtc tttcgcattt 7141 cgccctatat ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc 7201 cttcggcgcg gctccccctc cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc 7261 gcggccttcg gccttgccca aggtggcgct gcccccttgg aacccccgca ctcgccgccg 7321 tgaggctcgg ggggcaggcg ggcgggcttc gcccttcgac tgcccccact cgcataggct 7381 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc 7441 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt 7501 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg 7561 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg 7621 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag 7681 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag 7741 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg ggggcgcgcc cagctgtcta 7801 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa 7861 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ct //